The protein folding ‘speed limit’
نویسندگان
چکیده
منابع مشابه
The protein folding 'speed limit'.
How fast can a protein possibly fold? This question has stimulated experimentalists to seek fast folding proteins and to engineer them to fold even faster. Proteins folding at or near the speed limit are prime candidates for all-atom molecular dynamics simulations. They may also have no free energy barrier, allowing the direct observation of intermediate structures on the pathways from the unfo...
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Recent observations of fast steps in protein folding (1-3) address the following question: what is the shortest time that would be required for folding? The paper by Hagen et al. (4) in this issue of the Proceedings provides strong experimental support for a speed limit that is governed by diffusion. Diffusion is known to limit the rates of many of the activities of proteins, including enzymati...
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As the simplest and most prevalent motif of protein folding, α-helix initiation is the starting point of macromolecular complexity. In this work, helix initiation was directly measured via ultrafast temperature-jump spectroscopy on the smallest possible helix nucleus for which only the first turn is formed. The rate's dependence on sequence, length, and temperature reveals the fastest possible ...
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Current questions in protein folding mechanisms include how fast can a protein fold and are there energy barriers for the folding and unfolding of ultrafast folding proteins? The small 3-helical engrailed homeodomain protein folds in 1.7 μs to form a well-characterized intermediate, which rearranges in 17 μs to native structure. We found that the homologous pituitary-specific transcription fact...
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A direct measure of intramolecular chain diffusion is obtained by the determination of triplet-triplet energy-transfer rates between a donor and an acceptor chromophore attached at defined points on a polypeptide chain. Single exponential kinetics of contact formation are observed on the nanosecond time scale for polypeptides in which donor and acceptor are linked by repeating units of glycine ...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2004
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2004.01.013